On the distribution and habitat use of the sub-Antarctic fly Hyadesimyia clausa Bigot (Diptera, Tachinidae) according to citizen science.

Hyadesimyia clausa Bigot is a morphologically striking tachinid that inhabits the Sub-Antarctic Ecoregion of the Magallanes Region in Chile and Tierra del Fuego province in Argentina. Much of the distributional information about this species is restricted to the Cape Horn islands, which have extreme environmental conditions, but the species' natural history, range limits, and habitat use have never been described or confirmed. Our goals were to describe the distributional limits of this sub-Antarctic fly with the help of citizen science and use this information type to describe this tachinid's habitat use and potential biological interactions with nonvascular and vascular flora. We found that citizen science significantly increased our understanding of the extent of occurrence, expanding the known distributional range by 195 km to the north and 153 km to the west. On the contrary, the values for the area of occupancy were not significant, but the occupancy overlap between different records was very low. We confirmed that H. clausa's habitat uses peatlands and although we have not provided evidence of pollination or movement of spores, we hypothesized, that the walking activity of H. clausa could help move sperm from mosses and pollen from the flowers of vascular plants, so they could act as potential pollinators. Citizen science can reduce and eliminate some scientific knowledge shortfalls and propose new ecological questions that could increase our knowledge of extreme ecosystems.

Unraveling biotypes of the northern house mosquito, Culex pipiens s.l. (Diptera: Culicidae): molecular differentiation and morphometric analysis.

Geometric morphometrics was used to determine whether geographic isolation could explain differences in wing size and shape between and within continental (27°S to 41°S) and insular (Rapa Nui) populations of Culex pipiens s.s. Linnaeus and their biotypes (f. pipiens and f. molestus). Molecular protocols based on polymorphisms in the second intron of nuclear locus ace-2 (acetylcholinesterase-2) were used to differentiate Cx. pipiens s.s. from Cx. quinquefasciatus Say, and an assay based on polymorphisms in the flanking region of a microsatellite locus (CQ11) was used to identify biotypes. Culex pipiens f. molestus and hybrids shared larval habitats in all continental sites, while Cx. pipiens f. pipiens was found in 5 of the 10 sites. Only biotype molestus was found in Rapa Nui (Easter Island) Pipiens and molestus biotypes occur sympatrically in aboveground locations, and only molestus was found in the underground site (ME). Biotype molestus was dominant in rural locations and preferably anthropophilic. These results agree with the ecological descriptions previously reported for the biotypes of Cx. pipiens s.s. Procrustes ANOVA only showed differences in centroid size between biotypes in females and males and did not show significant differences in wing shape. However, we found significant differences among the geographic areas in the centroid size and wing shape of both females and males. Particularly, the population of Rapa Nui Island had shorter wings than the continental populations. The results highlight the effects of geographic and environmental processes on morphotypes in vector mosquitoes.

On the taxonomic status of Agelanius antenninus (Philip) (Diptera: Tabanidae) from Peru based upon evidence from a morphological analysis of the holotype.

The taxonomic status of Agelanius antenninus (Philip, 1969) was evaluated on the basis of a morphological study of the holotype, described from Huánuco, Peru. Results of analyses of A. antenninus and its morphological comparison with A. meridianus Rondani, the type-species of Agelanius Rondani, proved that A. antenninus does not belong to the genus Agelanius. The subgenus Styphocera Philip is reinstated as a valid subgenus of Stypommisa Enderlein. Thus, the generic combination Stypommisa (Styphocera) antennina Philip should be reinstated for this species.

Revalidation of Phalangomyia Dyar & Knab as a subgenus of Culex L. (Diptera: Culicidae) based on morphological and molecular evidence.

Culex apicinus Philippi, 1865 and its synonyms, Phalangomyia debilis Dyar & Knab, 1914, Cx. escomeli Brèthes, 1920 and Cx. alticola Martini, 1931, were re-examined employing molecular and morphological phylogenetic methods. Archival and fresh specimens were used for morphological diagnosis and to generate fragments of two single-copy nuclear genes (CAD, HB) and one mitochondrial gene (COI). Additional sequences of Cx. apicinus and species of the subgenus Culex of Culex Linnaeus, 1758 and other subgenera of the genus were obtained from GenBank for molecular analysis. Diagnostic morphological characteristics of Cx. apicinus and other species of the subgenus Culex were contrasted. Phylogenetic relationships were generated using DNA sequences of protein coding genes under both Maximum Likelihood and Bayesian approaches. Culex apicinus showed unique morphological traits that allow its classification in a separate subgenus. The subspecific status is supported by its recovery as a monophyletic clade within the genus Culex based on morphological and molecular characters. The synonymous Phalangomyia debilis provides an available subgeneric name. Consequently, Phalangomyia Dyar & Knab, 1914 is resurrected from synonymy with Culex Linnaeus, 1758 as a monobasic subgenus of Culex.

Northern and central Chile still free of emerging flaviviruses in mosquitoes (Diptera: Culicidae).

Geographic isolation and strict control limits in border areas have kept Chile free from various pathogens, including Flavivirus. However, the scenario is changing mainly due to climate change, the reintroduction of more aggressive mosquitoes, and the great wave of migration of people from endemic countries in recent years. Hence, it is necessary to surveillance mosquitoes to anticipate a possible outbreak in the population and take action to control it. This study aimed to investigate the presence of Flavivirus RNA by molecular tools with consensus primers in mosquitoes collected in the extreme north and central Chile. From 2019 to 2021, a prospective study was carried out in localities of Northern and part of Central Chile. Larvae, pupae, and adults of mosquitoes were collected in rural and urban sites in each locality. The collected samples were pooled by species and geographical location and tested using RT-PCR and RT-qPCR to determine presence of Flavivirus. 3085 specimens were collected, the most abundant specie Culex quinquefasciatus in the North and Aedes (Ochlerotatus) albifasciatus in the Center of Chile. Both genera are associated with Flavivirus transmission. However, PCR and RT-PCR did not detect Flavivirus RNA in the mosquitoes studied. These negative results indicate we are still a free Flavivirus country, which is reaffirmed by the non-existence of endemic human cases. Despite this, routine surveillance of mosquitoes and the pathogens they carry is highly recommended to evaluate each area-specific risk of vector-borne transmission.

Citizen Science Meet South American Tachinids: New Records of Feather-Legged Fly Trichopoda (Galactomyia) pictipennis Bigot (Diptera: Tachinidae) from Chile.

The species of Gymnosomatini (Diptera: Tachinidae) are specialized to attack stink bugs (Hemiptera: Pentatomidae). Some of these flies, such as those in the genus Trichopoda Berthold, are conspicuous and represent target species to study with citizen science. Here we report for the first time the distribution range of T. (Galactomyia) pictipennis Bigot, a newly introduced biological control species of tachinid for use against stink bugs in Chile using data obtained through the citizen science method. Additionally, we update the distribution of T. arcuata (Bigot), a native biological control species of tachinid, and assess the grade of overlap between the flies and their main stink bug hosts. We obtained data regarding the occurrence of flies and stink bugs from the citizen science program "Moscas Florícolas de Chile," from Facebook groups, and from iNaturalist. We standardized our data to avoid temporal, spatial, and geographic biases. We report the extent of occurrence (EOO) and area of occupancy (AOO) for each fly and stink bug species. The EOOs for T. pictipennis and T. arcuata are 85,474 km2 and 20,647 km2 through central Chile ecosystems, respectively. The EOO of T. arcuata is overlapped by 53% with the introduced species. Trichopoda pictipennis has a high percentage of overlap with the EOO of Nezara viridula Linnaeus (61%), whereas T. arcuata has low percentages of overlap with different native Acledra species and with N. viridula. We discuss the potential of citizen science to detect (1) areas with higher diversity or gaps of occurrences, (2) new biological control agents, (3) prey records, (4) negative impacts on non-target species, (5) changes in composition at long-term, and (6) areas to promote conservation biological control in agricultural landscapes.

Filarial nematodes in domestic dogs and mosquitoes (Diptera: Culicidae) from semi-rural areas in Central Chile.

Climate change, competent vectors, and reservoir animals are the main factors for developing vector-borne zoonotic diseases. These diseases encompass a significant and widespread category of pathogens (e.g., viruses, bacteria, protozoa, and helminths) transmitted by blood-feeding arthropods, including ticks, fleas, lice, triatomines, mosquitoes, sandflies, and blackflies. In Chile, several studies have explored the role of dogs as reservoirs of vector-borne pathogens; however, there is a lack of research investigating the presence of pathogens in arthropods. Specifically, within the order Diptera, limited knowledge exists regarding their roles as carriers of pathogens. This study aimed to examine the presence of zoonotic filarial nematodes in mosquitoes and dogs within a previously unstudied semi-rural area of Central Chile. Two hundred samples of dog blood and seven hundred and twenty-four mosquitoes were collected during 2021-2022 and studied for filarial nematodes by PCR. The prevalence of microfilaremic dogs detected by Knott's test was 7.5%, with Acanthocheilonema reconditum being the only species identified. Aedes (Ochlerotatus) albifasciatus was the most abundant mosquito species collected, and 15 out of 65 pools were positive for filarial nematodes. Among these pools, 13 tested positive for Acanthocheilonema reconditum, and two tested positive for Setaria equina through PCR. Additionally, five Culex pipiens specimens were positive for Acanthocheilonema reconditum. Despite the absence of zoonotic filarial species, these findings underscore the significance of monitoring pathogens in mosquitoes and animal hosts and continued research into the dynamics of vector-borne diseases, particularly in unexplored regions.

Chagas disease in northern Chile: Detection of Trypanosoma cruzi in children, dogs and triatomine bugs.

Chagas disease is an anthropozoonotic disease caused by the protozoan Trypanosoma cruzi, transmitted by triatomine vectors. In Chile, there are four species of triatomine bugs that are potential vectors of T. cruzi, being Triatoma infestans the main vector in endemic areas of the country. The "Programa Nacional de Control Vectorial de la Enfermedad de Chagas de Chile" has significantly reduced the rates of home infestation to less than 1% and has interrupted vectorial transmission since 1999. The objective of this study was to evaluate the impact of vectorial control and the continuity of the interruption of vectorial transmission in northern Chile (provincia de El Loa, región de Antofagasta). The study comprised fingerstick blood samples of 2104 children, attending local school, venous blood samples of 65 dogs, associated to houses with T. infestans unique findings and vector infestation, and intestine samples of 284 T. infestans specimens, from the provincia de El Loa, during 2014-2016 period. The samples were analyzed by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence and/or polymerase chain reaction (PCR) techniques. A total of 5 children (0.24%), 7 dogs (10.8%), and 6 specimens of T. infestans (2.1%) resulted positive to T. cruzi infection. This study showed that the risk of transmission of Chagas disease is low in the north of Chile (provincia de El Loa), detected a low positive rate of chagasic children and of infected triatomine bugs, and showed the existence of T. cruzi transmission in dogs, which are used as natural sentinels for the detection of T. cruzi infection, being especially useful during surveillance program in human population characterized by low seroprevalence.

Improving geographic distribution data for a putatively extinct species, a test case with a disappeared fly.

Global biodiversity loss by anthropogenic impacts is an under-recognized form of global environmental change. Global defaunation is still poorly documented in the case of insects, showing a significant decrease in populations and diversity. The blowfly Neta chilensis (Walker 1837) is poorly known and presumed to be confined to southern-South America, with an unclear distributional pattern. It was last collected in 1984. We aimed, through Ecological Niche Models, to identify regions highly suitable for N. chilensis; to test the suitability of regions with doubtful records; to understand the impact that climatic change and human activities have had; and to identify regions with high chances to find it. We compiled 130 presence records from Argentina and Chile and 117 localities where it was sought but not found between 1987 and 2018. Results indicate that suitable conditions are restricted to southern and central Chile and to southwestern Argentina, that doubtful records are predicted in unsuitable areas, that N. chilensis occupies a narrow niche and that its decline is not mainly caused by climate changes but more probably to habitat loss and to the biological invasion. We identified two regions where the chances of finding the species are higher in the case that it is not extinct already.

Blood-Meal Sources and Trypanosoma cruzi Infection in Coastal and Insular Triatomine Bugs from the Atacama Desert of Chile.

Mepraia parapatrica is one of the lesser known and less abundant sylvatic triatomine species naturally infected by the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. M. parapatrica lives in sympatry with T. cruzi-infected rodents, but only birds, reptiles, and marine mammals have been reported as blood-meal sources of this vector species by serology. The distribution range of this kissing bug overlaps with fishers' settlements and tourist areas, and therefore the study of the blood-meal sources of this triatomine species is relevant. Here, we determined the blood-meal sources of M. parapatrica by NGS or standard sequencing from a coastal mainland area and an island in northern Chile, and T. cruzi infection by real-time PCR. The blood-meals of. M parapatrica included 61.3% reptiles, 35.5% mammals (including humans) and 3.2% birds. Feeding on reptiles was more frequent on the mainland, while on the island feeding on mammals was more frequent. The presence of T. cruzi-infected triatomine bugs and humans as part of the diet of M. parapatrica in both areas represents an epidemiological threat and potential risk to the human population visiting or established in these areas. Currently there are no tools to control wild triatomines; these results highlight the potential risk of inhabiting these areas and the necessity of developing information campaigns for the community and surveillance actions.

Review of Homalocnemidae of Chile with a key to species and descriptions of male and female terminalia (Diptera: Empidoidea).

The Homalocnemidae of Chile is reviewed, including the descriptions of the male and female terminalia of the two known species: H. nigripennis Philippi and H. praesumpta Collin. All references pertaining to Chilean Homalocnemis Philippi and the two known species are provided. Geographical distributions of the species were compiled and mapped from bibliographic data and collections. An illustrated key to the two species is also presented.

The emergence of Dirofilaria repens in a non-endemic area influenced by climate change: dynamics of transmission using a mathematical model.

Dirofilaria repens is a nematode affecting domestic and wild canids, transmitted by several species of mosquitoes of different genera. It usually causes a non-pathogenic subcutaneous infection in dogs and is the principal agent of human dirofilariasis in the Old World. The geographic distribution of D. repens is changing rapidly, and several factors contribute to the spread of the infection to non-endemic areas. A mathematical model for transmission of Dirofilaria spp. was built, using a system of ordinary differential equations that consider the interactions between reservoirs, vectors, and humans. The transmission simulations of D. repens were carried out considering a projection in time, with intervals of 15 and 100 years. For the dynamics of the vector, seasonal variations were presented as series with quarter periodicity during the year. The results of the simulations highlight the peak of contagions in the reservoir and in humans, a product of the action of the vector when it remains active throughout the year. A 300% infection increase in the reservoir was observed during the first decade and remains present in the population with a representative number of cases. When the vector maintains its density and infectivity during the year, the incidence of the infection in humans increases. Accumulated cases amount to 45 per 100,000 inhabitants, which corresponds to a cumulative incidence of 0.05%, in 85 years. This indicates that early prevention of infection in canids would significantly reduce the disease, also reducing the number of accumulated cases of human dirofilariasis by D. repens. The interaction between the simulations generated by the model highlights the sensitivity of the epidemiological curve to the periodicity of seasonality, reaffirming the hypothesis of the probability of movement of the zoonotic disease to non-endemic areas, due to climate change.

Annotated catalogue of the Tachinidae (Insecta, Diptera) of Chile.

The Tachinidae (Diptera) of Chile are catalogued and information is given on distributions, name-bearing types, synonyms, nomenclatural issues, and pertinent literature. The history of tachinid collectors in Chile and authors who have contributed to the systematic knowledge of Chilean tachinids is extensively reviewed. The classification has been updated and 122 genera and 264 species are recognised in Chile. There is a significant amount of endemism with 28 genera and 100 species known only from Chile. There are also 113 species with distributions shared only between Chile and Argentina, particularly in the southern portions of these countries comprising Patagonia. The catalogue is based on examination of the original descriptions of all nominal species and all other references known to us containing relevant taxonomic and distributional information, for a total of approximately 450 references. Many of the name-bearing types and other Chilean specimens housed in collections were examined. Taxa are arranged hierarchically and alphabetically under the categories of subfamily, tribe, genus, subgenus (where recognised), and species. Nomenclatural information is provided for genus-group and species-group names, including lists of synonyms (mostly restricted to Neotropical taxa) and name-bearing type data. Species distributions are recorded by country within the New World and by larger geographical divisions in the Old World. Additional information is given in the form of notes and references under valid names at the level of tribe, genus, and species. Two genera are newly recorded from Chile: Chaetoepalpus Vimmer & Soukup, 1940 (Tachinini) (also newly recorded from Argentina) and Patelloa Townsend, 1916 (Goniini). Four species are newly recorded from Chile or other countries: Lyphaornata Aldrich, 1934 (Chile); Chaetoepalpuscoquilleti Vimmer & Soukup, 1940 (Argentina and Chile); Phytomypteraevanescens (Cortés, 1967) (Argentina); and Xanthobasisunicolor Aldrich, 1934 (Chile). Eight species previously recorded from Chile are deemed to have been misidentified or misrecorded from Chile (known distributions in parentheses): Archytasincertus (Macquart, 1851) (Argentina, Brazil, Paraguay, Uruguay); Archytasseminiger (Wiedemann, 1830) (Brazil, Colombia); Goniacrassicornis (Fabricius, 1794) (Brazil, Peru, Venezuela, Middle America, West Indies, Nearctic); Lespesiaandina (Bigot, 1888) (Cuba); Lespesiaarchippivora (Riley, 1871) (widespread Nearctic and most of Neotropical); Neoethillaignobilis (van der Wulp, 1890) (Mexico, United States); Siphona (Siphona) geniculata (De Geer, 1776) (Palaearctic, Nearctic [introduced]); and Winthemiaquadripustulata (Fabricius, 1794) (Palaearctic, Nearctic, Oriental]. As First Reviser we fix Paratheresiarufiventris Townsend, 1929 as the senior homonym and Sarcoprosenarufiventris Townsend, 1929 as the junior homonym when the two are placed together in Billaea Robineau-Desvoidy, 1830; and we fix Mayophoriniaangusta Townsend, 1927 as the senior homonym and Metarrhinomyiaangusta Townsend, 1927 as the junior homonym when the two are placed together in Myiopharus Brauer & Bergenstamm, 1889. New replacement names are proposed for eight preoccupied names of Neotropical species (country of type locality in parentheses): Billaearufescens O'Hara & Wood for Sarcoprosenarufiventris Townsend, 1929, preoccupied in the genus Billaea Robineau-Desvoidy, 1830 by Paratheresiarufiventris Townsend, 1929 (Peru), nom. nov.; Billaeatriquetrus O'Hara & Wood for Sarcoprosenatriangulifera Townsend, 1927, preoccupied in the genus Billaea Robineau-Desvoidy, 1830 by Dexiatriangulifera Zetterstedt, 1844 (Peru), nom. nov.; Eucelatorianudioculata O'Hara & Wood for Eucelatorioideanigripalpis Thompson, 1968, preoccupied in the genus Eucelatoria Townsend, 1909 by Chetolyganigripalpis Bigot, 1889 (Trinidad), nom. nov.; Eucelatoriaoblonga O'Hara & Wood for Urodexodeselongatum Cortés & Campos, 1974, preoccupied in the genus Eucelatoria Townsend, 1909 by Exoristaelongata van der Wulp, 1890 (Chile), nom. nov.; Lespesiathompsoni O'Hara & Wood for Sturmiopsoideaobscura Thompson, 1966, preoccupied in the genus Lespesia Robineau-Desvoidy, 1863 by Eurigasterobscurus Bigot, 1857 (Cuba), nom. nov.; Myiopharuscharapensis O'Hara & Wood for Metarrhinomyiaangusta Townsend, 1927, preoccupied in the genus Myiopharus Brauer & Bergenstamm, 1889 by Mayophoriniaangusta Townsend, 1927 (Peru), nom. nov.; Myiopharusincognitus O'Hara & Wood for Stenochaetaclaripalpis Thompson, 1968, preoccupied in the genus Myiopharus Brauer & Bergenstamm, 1889 by Neoxynopsoideaclaripalpis Thompson, 1968 (Trinidad), nom. nov.; and Myiopharusrufopalpus O'Hara & Wood for Paralispepalpalis Townsend, 1929, preoccupied in the genus Myiopharus Brauer & Bergenstamm, 1889 by Myioxynopspalpalis Townsend, 1927 (Peru), nom. nov. New type species fixations are made under the provisions of Article 70.3.2 of the ICZNCode for three genus-group names: Parafabricia Brauer & Bergenstamm, 1894 (synonym of Archytas Jaennicke, 1867), type species newly fixed as Parafabriciaperplexa Townsend, 1931; Tachinodes Brauer & Bergenstamm, 1889 (synonym of Archytas Jaennicke, 1867), type species newly fixed as Juriniametallica Robineau-Desvoidy, 1830; and Willistonia Brauer & Bergenstamm, 1889 (synonym of Belvosia Robineau-Desvoidy, 1830), type species newly fixed as Willistoniaaldrichi Townsend, 1931. Lectotypes are designated for the following four nominal species, all described or possibly described from Chile: Echinomyiapygmaea Macquart, 1851 (a valid name in the genus Peleteria Robineau-Desvoidy, 1830); Goniachilensis Macquart, 1844 (a junior synonym of Goniapallens Wiedemann, 1830); Masiceraauriceps Macquart, 1844 (a valid name in the genus Lespesia Robineau-Desvoidy, 1863); and Prosopochoetanitidiventris Macquart, 1851 (a valid name in the genus Prosopochaeta Macquart, 1851). The following 27 new or revived combinations are proposed (distributions in parentheses): Blepharipezaandina Bigot, 1888 is moved to Lespesia Robineau-Desvoidy, 1863 as L.andina, nomen dubium (Cuba), comb. nov.; Camposodesevanescens Cortés, 1967 is moved to Phytomyptera Rondani, 1845 as P.evanescens (Argentina, Chile), comb. nov.; Ectophasiopsisypiranga Dios & Nihei, 2017 is moved to Trichopoda Berthold, 1827 and assigned to subgenus Galactomyia Townsend, 1908 as T. (G.) ypiranga (Argentina, Brazil), comb. nov.; Embiomyiaaustralis Aldrich, 1934 is moved to Steleoneura Stein, 1924 as S.australis (Argentina, Chile), comb. nov.; Eurigastermodestus Bigot, 1857 is moved to Lespesia as L.modesta (Cuba), comb. nov.; Eurigasterobscurus Bigot, 1857 is moved to Lespesia as L.obscura (Cuba), comb. nov.; Macropatelloatanumeana Townsend, 1931 is moved to Patelloa Townsend, 1916 as P.tanumeana (Argentina, Chile), comb. nov.; Masicerainsignis van der Wulp, 1882 is moved to Drino Robineau-Desvoidy, 1863 as D.insignis (Argentina, Chile), comb. nov.; Parasetigenahichinsi Cortés, 1967 is moved to Chetogena Rondani, 1856 as C.hichinsi (Chile), comb. nov.; Parasetigenaporteri Brèthes, 1920 and junior synonym Stomatotachinasplendida Townsend, 1931 are moved to Chetogena as C.porteri (Chile), both comb. nov.; Phoroceracalyptrata Aldrich, 1934 is moved to Admontia Brauer & Bergenstamm, 1889 as A.calyptrata (Argentina, Chile), comb. nov.; Poliopsauratus Campos, 1953 is moved to Admontia Brauer & Bergenstamm, 1889 as A.aurata (Chile), comb. nov.; Poliopsstriatus Aldrich, 1934 is moved to Admontia as A.striata (Argentina, Chile), comb. nov.; Ruiziellafrontosa Cortés, 1951 is moved to Chaetoepalpus Vimmer & Soukup, 1940 and placed in synonymy with C.coquilleti Vimmer & Soukup, 1940 (Argentina, Chile, Peru), comb. nov.; Ruiziellaluctuosa Cortés, 1951 is moved to Chaetoepalpus as C.luctuosus (Argentina, Chile), comb. nov.; Sarcoprosenaluteola Cortés & Campos, 1974 is moved to Billaea Robineau-Desvoidy, 1830 as B.luteola (Chile), comb. nov.; Sarcoprosenarufiventris Townsend, 1929 is moved to Billaea where it is a junior secondary homonym and is renamed B.rufescens O'Hara & Wood (Peru), comb. nov.; Sarcoprosenatriangulifera Townsend, 1927 is moved to Billaea where it is a junior secondary homonym and is renamed B.triquetrus O'Hara & Wood (Peru),comb. nov.; Saundersiaaurea Giglio-Tos, 1893 is moved to "Unplaced species of Tachinini" (Mexico), comb. nov.; Schistostephanaaurifrons Townsend, 1919 is moved to Billaea as B.aurifrons (Peru), comb. nov.; Siphoactiacharapensis Townsend, 1927 is moved to Clausicella Rondani, 1856 as C.charapensis (Peru), comb. nov.; Siphoactiaperegrina Cortés & Campos, 1971 is moved to Clausicella as C. peregrina (Chile), comb. nov.; Sturmiafestiva Cortés, 1944 is moved to Drino as D.festiva (Argentina, Chile), comb. nov.; Sturmiopsoideaobscura Thompson, 1966 is moved to Lespesia Robineau-Desvoidy, 1863, where it is a junior secondary homonym and is renamed L.thompsoni O'Hara & Wood (Trinidad), comb. nov.; Trichopodaarcuata Bigot, 1876 is returned to Trichopoda from Ectophasiopsis Townsend, 1915 and assigned to subgenus Galactomyia (Argentina, Chile), comb. revived; and Trichopodagradata Wiedemann, 1830 is returned to Trichopoda from Ectophasiopsis and assigned to subgenus Galactomyia (Argentina, Brazil, Uruguay), comb. revived. New or revived generic and specific synonymies are proposed for the following 14 names: Camposodes Cortés, 1967 with Phytomyptera Rondani, 1845, syn. nov.; Ectophasiopsis Townsend, 1915 with Trichopoda Berthold, 1827, subgenus Galactomyia Townsend, 1908, syn. nov.; Embiomyia Aldrich, 1934 with Steleoneura Stein, 1924, syn. nov.; Fabriciaandicola Bigot, 1888 with Peleteriarobusta (Wiedemann, 1830), syn. revived; Macropatelloa Townsend, 1931 with Patelloa Townsend, 1916, syn. nov.; Peleteriainca Curran, 1925 with Peleteriarobusta (Wiedemann, 1830), syn. revived;Poliops Aldrich, 1934 with Admontia Brauer & Bergenstamm, 1889, syn. nov.; Ruiziella Cortés, 1951 with Chaetoepalpus Vimmer & Soukup, 1940, syn. nov.; Ruiziellafrontosa Cortés, 1951 with Chaetoepalpuscoquilleti Vimmer & Soukup, 1940, syn. nov.; Sarcoprosena Townsend, 1927 with Billaea Robineau-Desvoidy, 1830, syn. nov.; Schistostephana Townsend, 1919 with Billaea, syn. nov.; Siphoactia Townsend, 1927 with Clausicella Rondani, 1856, syn. nov.; Stomatotachina Townsend, 1931 with Chetogena Rondani, 1856, syn. nov.; and Sturmiopsoidea Thompson, 1966 with Lespesia Robineau-Desvoidy, 1863, syn. nov.

A catalog of the Brachystomatidae of Chile (Diptera: Empidoidea).

A catalog of the Brachystomatidae of Chile is provided. We present all valid names and synonyms for the 36 species and five genera distributed in the country, including information about name, author, year of publication, page number, type species, type depository, type locality and references. Heterophlebus Philippi is the richest genus in Chile, with 16 species, followed by Apalocnemis Philippi with 11 species, Ceratomerus Philippi with seven and Gondwanamyia Sinclair, Cumming, Brooks, Plant Saigusa and Hyperperacera Collin with one species each. The geographical distribution of the different species was compiled from bibliographic data and revised collections.

Modeling the Potential Distribution of the Malaria Vector Anopheles (Ano.) pseudopunctipennis Theobald (Diptera: Culicidae) in Arid Regions of Northern Chile.

The extreme north of Chile presents a subtropical climate permissive of the establishment of potential disease vectors. Anopheles (Ano.) pseudopunctipennis is distributed from the south of the United States to the north of Argentina and Chile, and is one of the main vectors of malaria in Latin America. Malaria was eradicated from Chile in 1945. Nevertheless, the vector persists in river ravines of the Arica and Tarapacá regions. The principal effect of climate change in the north of Chile is temperature increase. Precipitation prediction is not accurate for this region because records were erratic during the last century. The objective of this study was to estimate the current and the projected distribution pattern of this species in Chile, given the potential impact due to climate change. We compiled distributional data for An. (Ano.) pseudopunctipennis and constructed species distribution models to predict the spatial distribution of this species using the MaxEnt algorithm with current and RCP 4.5 and 8.5 scenarios, using environmental and topographic layers. Our models estimated that the current expected range of An. (Ano.) pseudopunctipennis extends continuously from Arica to the north of Antofagasta region. Furthermore, the RCP 4.5 and 8.5 projected scenarios suggested that the range of distribution of An. (Ano.) pseudopunctipennis may increase in longitude, latitude, and altitude limits, enhancing the local extension area by 38 and 101%, respectively, and local presence probability (>0.7), from the northern limit in Arica y Parinacota region (18°S) to the northern Antofagasta region (23°S). This study contributes to geographic and ecologic knowledge about this species in Chile, as it represents the first local study of An. (Ano.) pseudopunctipennis. The information generated in this study can be used to inform decision making regarding vector control and surveillance programs of Latin America. These kinds of studies are very relevant to generate human, animal, and environmental health knowledge contributing to the "One Health" concept.

Buestanmyia chiriboga gen. nov. et sp. nov. (Diptera: Tabanidae: Diachlorini) from the Ecuadorian Andes.

Buestanmyia gen. nov., is described from Pichincha Province, Ecuador with one included species, with B. chiriboga sp. nov. It is characterized by very short and scarce ommatrichia, frons wide and slightly divergent below, basal callus subquadrate with median dorsal projection reaching half the height of the frons, ocellar triangle and ocelli absent, scape 3.5x the length of the pedicel, scape and pedicel with strong and erect hairs, proboscis less than the length of the head, basicosta bare, wings slightly smoky, with clouds on the cells costal and subcostal, vein R4 with a short appendix; cell cup with short appendix.

A catalog of Pelecorhynchidae (Diptera: Tabanomorpha) from Chile.

A catalog of the Pelecorhynchidae from Chile is provided. We presented all valid names and synonyms for the 9 species and 1 genus for the country, including information about name, author, year of publication, page number, type species, type locality and references. The geographical distribution of the species known from Chile was compiled from bibliographic data and revised collections.

A catalog of the Bibionidae (Diptera: Bibionomorpha) of Chile.

A catalog of the Bibionidae of Chile is provided. We present all valid names and synonyms for the 33 species and 2 genera known from the country, including information about name, author, year of publication, page number, type species, type locality and references. The geographical distribution of the species known from Chile was compiled from bibliographic data and revised collections. Epiplecia Giard, Heteroplecia Hardy, and Pleciodes Hardy are proposed as junior synonyms of Plecia Wiedeman.

A catalog of Rhagionidae (Diptera: Brachycera) from Chile.

The genera and species of Chilean rhagionids (Diptera: Rhagionidae) are listed, with annotated references to nomenclature, synonymies and generic combinations, type localities, the primary type depositories, distribution, and citations for the most recent revisions. The genus Atherimorpha White is the richest genus in Chile, with 15 described species. Only Leptis basalis Philippi, 1865 is kept as incertae sedis in the family.

A catalog of Athericidae (Diptera) from Neotropical and Andean Regions.

A catalog of the Athericidae from Neotropical and Andean Regions is provided. All valid names and synonyms are presented, totaling twenty-seven species and three genera in these Regions. All references known from the taxonomic and biological literature are given, including information about name, author, year of publication, page number, type species, type locality and bibliography. The genus Atherix Meigen is not represented in Neotropical nor Andean Regions.